欧美呦交-欧美永久免费-欧美影视一区-欧美伊人久久大香线蕉综合69-97成人精品-97se亚洲综合自在线尤物

資料下載

Data download

當(dāng)前位置:首頁資料下載質(zhì)構(gòu)儀相關(guān)SCI論文—蛋殼硬度測(cè)定

質(zhì)構(gòu)儀相關(guān)SCI論文—蛋殼硬度測(cè)定

發(fā)布時(shí)間:2021/9/6點(diǎn)擊次數(shù):1244

Abstract

The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modifiedmutant insects. In the construction of CRISPR/Cas9-mediated dsLmRNase2–/– mutant locusts, we found that the tanned eggs which experienced a 20-min contact with the oocyst exhibited a higher success rate compared to fresh newlylaid eggs that were less tanned. However, the heritable efficiency of the dsLmRNase2 deletion to the next generation G1 progeny was similar between adults derived from the tanned or less tanned engineered eggs. Further, the similar effective mutant ratios in the normally developed eggs and G0 adults of tanned and less tanned eggs also indicated that tanning did not reduce the absolute mutation efficiency induced by CRISPR/Cas9. Moreover, we found that the syncytial division period, which was longer than the time for tanning, conferred a window period for microinjection treatment with efficient mutation in both tanned and less tanned eggs. We further found that tanned eggs exhibited a higher hatching rate due to a reduced infection rate following microinjection. Both the anti-pressure and ultrastructure analyses indicated

that the tanned eggs contained compressed eggshells to withstand increased external pressure. In summary, tanned eggs possess stronger defense responses and higher efficiency of genome editing, providing an improved model for developing Cas9-mediated gene editing procedures in locusts.


文件下載    
服務(wù)熱線

021-54397372

15618141270

15300622190

添加微信

返回頂部

點(diǎn)



主站蜘蛛池模板: 久久免费观看爱情动作片| 欧美精品亚洲精品日韩专区va| 真实处破女网站| 恶搞之家带颜色的剧集| 一区二区在线视频观看| 西瓜视频全部免费观看大全| 国产成人免费在线| www.99精品视频在线播放| www4虎| 九九自拍视频| 欧美日韩国产在线观看一区二区三区| 四虎影视在线影院4hu| 亚洲小视频在线观看| 在线一区视频| 欧美一区二区三区免费| jizzjizzjⅰzz亚洲美女| 久久久久久网| 亚洲精品国产高清嫩草影院| 黄污视频在线观看| 精品国产成人| 四虎看片| 国产porn| 日本电车痴汉成a人片| 日本动漫新番| 国产精品视频免费视频| 全部免费毛片免费播放| 日韩在线2020专区| 国产成人18黄网站免费网站| 青青免费在线视频| 天天操天天插天天干| 日韩久久影院| 四虎免费在线视频| 成人国内精品久久久久影院| 美日韩中文字幕| 午夜精品网| 久久精品中文字幕有码日本| 在线综合+亚洲+欧美中文字幕| 高清午夜线观看免费| 日韩欧美第一区二区三区| 欧美日韩免费一区二区在线观看| 最新高清无码专区|